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     EduGorilla 
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    Directions: The purification data for an enzyme is given below:
     

      Step Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg)
    P Cell-free extract 17 177 102 0.58
    Q Q-Sepharose 14 18.8 72 3.83
    R Phenyl Sepharose 26 9.2 45 4.89
    S Sephacryl S-200 7 4.1 30 7.32

    The fold purification for each step is

    Options :-

    1. P - 0.1, Q - 0.66, R - 0.84, S - 1.26
    2. P - 1.0, Q - 0.52, R - 0.67, S - 0.8
    3. P - 1, Q - 6.6, R - 8.4, S - 12.6
    4. P - 100, Q - 66, R - 84, S - 12
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