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     EduGorilla 
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    Biological nitrogen fixation (BNF), discovered by Beijerinck in 1901, is performed by a specialised group of organisms (prokaryotes). These prokaryotes use the enzyme nitrogenase to catalyze the process of transformation of atmospheric nitrogen (N2) to ammonia (NH3). Which of the following organisms can bring about biological nitrogen fixation?

    Options :-

    1. Cyanobacteria
    2. Azotobacter
    3. Azospirillium
    4. Rhizobium
    5. All of the above
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